Part:BBa_K3078013:Design

The mutation enhanced T7 promoter . A3->G3.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Mutated T7 promoter's intensity is weaker than the previous one (BBa_K2150031).

Source

We found the sequence from the paper DOI: 10.1007/s12010-019-03113-y.

References

[1] Ikeda R A, Warshamana G S, Chang L L. In vivo and in vitro activities of point mutants of the bacteriophage T7 RNA polymerase promoter[J]. Biochemistry, 1992, 31(37): 9073-80.

[2] Paul S, Stang A, Lennartz K, et al. Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution[J]. Nucleic Acids Res, 2013, 41(1): e29.

[3] Nie Z, Luo H, Li J, et al. High-Throughput Screening of T7 Promoter Mutants for Soluble Expression of Cephalosporin C Acylase in E. coli[J]. Appl Biochem Biotechnol, 2019